Last week, the photoactivation of neurons using optogenetics technique doesn't really work out. We have tried to use light with different frequencies and amplitude. However, there are no observable changes in the electroencephalogram(EEG) recording.
We then plan to check the expression of opsins with fluorescence imaging. The mouse model we are using is designed to express opsins in parvalbumin interneuron, which have a large population in layer 4 of the mouse brain. Thus, a fluorescence endoscope is built using a fiber bundle to image neurons that express fluoresce dye (td-Tomato). The fiber bundle is inserted into the brain to try to image those neurons. However, we suspect that the light source is not bright enough to image the fluorescence signal since we are only using LEDs as the light source. We would try to switch to laser source for a better imaging quality.
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