Week 5: Jerry got seizure

Our lab introduces seizure in a mouse with 2 different chemicals, 4-aminopyridine (4-AP) or FeCl3. 4-Ap is used to acute seizure introduction while FeCl3 is for chronic seizure introduction.

Since different dosage of 4-AP will introduce different amplitude of seizure, we are trying to try different dosage and concentration of the 4-AP to introduce a "good" seizure. Since the experiments in the lab are switching from using mice in anesthesia to awake mouse, we are exploring the best dosage for it.

A good seizure should have an abundant time between Ictal-seizure for better post-processing. If a high dosage of 4-AP is injected, a continuous Ictal-seizure will result and the mouse will die after a few minutes.

FeCl3, on the other hand, requires approximately 9 weeks for seizure to occur. It is used for a longitudinal study of the disease mice model.